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Ensure Comprehensive Protection and Stability for Your Precious Samples
Preserve Your RNA at Higher Temperatures
RNaseRIP Ribonuclease Inhibitor is a potent, rationally designed, chimeric ribonuclease (RNase) inhibitor featuring increased thermostability at 60°C (Figure 1) and oxidation resistance.
Know That Your RNA is Safe
RNaseRIP shows equivalent performance to other RNase inhibitors in the market (Figure 2).
RNaseRIP was able to protect the RNA found in a dirty sample and ensure a good signal in a real-time RT-PCR assay.
Even in low DTT conditions
RNaseRIP is more resistant to oxidative damage and continues to protect in the absence of Dithiothreitol (DTT) (Figure 3).
This makes RNaseRIP suitable for reactions where DTT may cause interference, such as quantitative real-time PCR (qPCR). DTT has been shown to interfere with a number of fluorescence dyes commonly used for qPCR (ref. 1, 2).
References:
1. Hudson et. al., 2021, J. Forensic Sci.
2. Boiso, 2015, Forensic Science Int. G.S.S.
Figure 1. Graph showing how RNaseRIP is more thermostable than existing RNase inhibitors. When the incubation temperature is raised to 60°C, RNaseRIP continues to offer protection against RNases in this fluorometric assay.
Figure 2. Graph showing that RNaseRIP can protect RNA samples from RNases. Virus was spiked into nasopharyngeal (NP) swabs and used for real-time reverse transcription PCR (RT-PCR) to detect viral gene N. No RNA extraction was done prior to running the assay. Having RNaseRIP present in the reaction resulted in a positive signal detected in all samples, comparable to a market-leading competitor.
Figure 3. Graph showing that RNaseRIP continues to protect RNA samples from RNases, even when DTT is absent. RNaseRIP Ribonuclease Inhibitor was added to reactions containing RNase A, with and without DTT added to the buffer (+RNaseRIP, +/- DTT). As long as RNaseRIP was present, reactions continued to enjoy a healthy level of protection against RNases in this fluorometric assay.
Product Overview
RNaseRIP Ribonuclease Inhibitor (RNaseRIP) is thermostable and oxidation resistant. It is therefore useful in any application where RNases could be a potential problem and is ideal for high DTT sensitive reactions (e.g. real-time PCR). RNaseRIP has been shown to function even in low DTT conditions and higher temperature.
RNaseRIP is an engineered, chimeric, recombinant protein expressed in E. coli.
RNaseRIP does not interfere with reactions containing AbScript 3.0 Reverse Transcriptase, M-MLV Reverse Transcriptase, AbTaq 2.0 Polymerase and Taq polymerase.
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