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RNaseRIP Ribonuclease Inhibitor
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Product Description
A potent, rationally designed chimeric ribonuclease inhibitor (RNase inhibitor) featuring increased thermostability between 55 to 60oC and oxidation resistance.
RNaseRIP Ribonuclease Inhibitor is useful in any application where RNases could be a potential problem and is ideal for high DTT-sensitive reactions (e.g. real-time PCR).
Catalog Number | Component Name | Size/Amount | Concentration |
---|---|---|---|
RR-RI-001-2500U | |||
RNase Ribonuclease Inhibitor | 2500U / 1 x 62.5uL | 40U/uL | |
RR-RI-001-5000U | |||
RNaseRIP Ribonuclease Inhibitor | 5000U / 1 x 125uL | 40U/uL | |
RR-RI-001-5000U-GF | |||
RNaseRIP Ribonuclease Inhibitor | 5000U / 1 x 125uL | 40U/uL | |
Features | Specifications |
---|---|
Concentration | 40U/uL |
Activity | Non-competitive binding to pancreatic-type ribonucleases. Does not inhibit modifying enzymes like reverse transcriptases. |
Stability | Thermostable and resistant to oxidation |
Unit Assay
The unit concentration of each lot is determined to be at least 40U/uL. Activity is measured by the inhibition of hydrolysis of cytidine 2’, 3’-cyclic monophosphate by RNase A.
RNase-Free
Each lot shows no detectable degradation of RNA when 40U of RNaseRIP Ribonuclease Inhibitor was incubated with 500ng of RNA ladder for 1 hour at 37°C as determined by agarose gel electrophoresis.
Protein Purity
Each lot is ≥90% pure as determined by SDS-PAGE analysis using Coomassie blue detection.