RNaseRIP Ribonuclease Inhibitor

RR-RI-001-2500U (2500U, Glycerol)

RR-RI-001-5000U (5000U, Glycerol)

RR-RI-001-5000U-GF (5000U, Glycerol-Free)

RR-RI-001-FS (Free Sample, Glycerol)

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Product Overview

A potent, rationally designed chimeric ribonuclease inhibitor (RNase inhibitor) featuring increased thermostability between 55 to 60oC and oxidation resistance.

RNaseRIP Ribonuclease Inhibitor is useful in any application where RNases could be a potential problem and is ideal for high DTT-sensitive reactions (e.g. real-time PCR).

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Catalog Number	Component Name	Size/Amount	Concentration
RR-RI-001-2500U
	RNase Ribonuclease Inhibitor	2500U / 1 x 62.5uL	40U/uL

RR-RI-001-5000U
	RNaseRIP Ribonuclease Inhibitor	5000U / 1 x 125uL	40U/uL

RR-RI-001-5000U-GF
	RNaseRIP Ribonuclease Inhibitor	5000U / 1 x 125uL	40U/uL

RR-RI-001-FS
	RNase Ribonuclease Inhibitor	200U / 1 X 5uL	40U/uL

Specifications and Features

Features	Specifications
Concentration	40U/uL
Activity	Non-competitive binding to pancreatic-type ribonucleases. Does not inhibit modifying enzymes like reverse transcriptases.
Stability	Thermostable and resistant to oxidation

Applications

cDNA synthesis, RT-PCR, and in vitro transcription and translation. It is useful in any application where RNases could be a potential problem.

Quality Controls

Unit Assay
The unit concentration of each lot is determined to be at least 40U/uL. Activity is measured by the inhibition of hydrolysis of cytidine 2’, 3’-cyclic monophosphate by RNase A.

RNase-Free
Each lot shows no detectable degradation of RNA when 40U of RNaseRIP Ribonuclease Inhibitor was incubated with 500ng of RNA ladder for 1 hour at 37°C as determined by agarose gel electrophoresis.

Protein Purity
Each lot is ≥90% pure as determined by SDS-PAGE analysis using Coomassie blue detection.