AbScript 3.0 Reverse Transcriptase

SP-RT-001-2000U (2000U, Glycerol)

SP-RT-001-10kU (10,000U, Glycerol)

SP-RT-001-10kU-GF (10,000U, Glycerol-Free)

SP-RT-001-40 (4 X 10,000U, Glycerol)

SP-RT-001-FS (Trial Pack, Glycerol)

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Product Overview

AbScript™ 3.0 Reverse Transcriptase is an enhanced Moloney Murine Leukemia Virus-derived (M-MLV) RNA-dependent DNA polymerase capable of high-quality long cDNA synthesis (> 9kDa), increased thermostability and reduced RNase H activity. Synthesis of cDNA is possible at temperatures up to 55℃.

Post reverse transcription, simply add part of the reaction mix to your real time PCR reaction (RT-PCR).

Independent RT-PCR tests by customers show that AbScript™ 3.0 Reverse Transcriptase is able to match the sensitivity of other commercial reverse transcriptases (RT).

Want to give our reagents a try? TRIAL samples available (>50% off our regular price). Add to cart now!
*Limited to one request per customer

Catalog Number	Component Name	Size/Amount	Concentration
SP-RT-001-FS
	AbScript 3.0 Reverse Transcriptase (Trial Pack)	1000U / 1 x 5uL	200U/uL
	5x AbScript Buffer	1 x 500uL	5x
	DTT	1 x 250uL	0.1M
SP-RT-001-2000U
	AbScript 3.0 Reverse Transcriptase	2000U / 1 x 10uL	200U/uL
	5x AbScript Buffer	1 x 1mL	5x
	DTT	1 x 0.5mL	0.1M
SP-RT-001-10kU
	AbScript 3.0 Reverse Transcriptase	10,000U / 1 x 50uL	200U/uL
	5x AbScript Buffer	1 x 1mL	5x
	DTT	1 x 0.5mL	0.1M
SP-RT-001-40
	AbScript 3.0 Reverse Transcriptase	4 x 10,000U / 4 x 50uL	200U/uL
	5x AbScript Buffer	4 x 1mL	5x
	DTT	4 x 0.5mL	0.1M
SP-RT-001-10kU-GF
	AbScript 3.0 Reverse Transcriptase	10,000U / 1 x 50uL	200U/uL
	5x AbScript Buffer	1 x 1mL	5x
	DTT	1 x 0.5mL	0.1M

Specifications and Features

Features	Specifications
Concentration	200U/uL
Recombinant Enzyme	Yes, an RNA dependent DNA polymerase
Enzyme Reaction	Reverse Transcription; first strand synthesis
Sample/Target	RNA
RNase H activity	Reduced
Included dNTPs	No
Reaction Condition	At 1x concentration the reaction buffer assures optimal activity for AbScript 3.0 Reverse transcriptase

Applications

RT-PCR, cDNA synthesis, qRT-PCR

Quality Controls

Unit Assay
The unit concentration of each lot has been determined to be at least 200U/uL

Specific Activity
The specific activity of each lot is at least 280,000 U/mg

RNase-Free
Each lot shows no detectable degradation of RNA when 200U of enzyme and 1x concentration of buffer was incubated with 500ng of RNA ladder for 1 hour at 37℃ as determined by agarose gel electrophoresis.

Protein Purity
Each lot is ≥95% pure as determined by SDS-PAGE analysis using Coomassie blue detection.

AbScript 3.0 Reverse Transcriptase Product Information.pdf

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